rabbit anti human scd1  (Proteintech)

Journal: Liver Research

Article Title: Combination of brefeldin A and tunicamycin induces apoptosis in HepG2 cells through the endoplasmic reticulum stress-activated PERK-eIF2α-ATF4-CHOP signaling pathway

doi: 10.1016/j.livres.2025.01.004

Figure Lengend Snippet: Antibodies used for Western blots.

Article Snippet: Rabbit anti-human SCD1 , Protein Tech, China , 28678-1-AP , 1:1000.

Techniques: Western Blot

Journal: Liver Research

Article Title: Combination of brefeldin A and tunicamycin induces apoptosis in HepG2 cells through the endoplasmic reticulum stress-activated PERK-eIF2α-ATF4-CHOP signaling pathway

doi: 10.1016/j.livres.2025.01.004

Figure Lengend Snippet: Primers of the genes used for qRT-PCR.

Article Snippet: Rabbit anti-human SCD1 , Protein Tech, China , 28678-1-AP , 1:1000.

Techniques: Sequencing

Journal: Liver Research

Article Title: Combination of brefeldin A and tunicamycin induces apoptosis in HepG2 cells through the endoplasmic reticulum stress-activated PERK-eIF2α-ATF4-CHOP signaling pathway

doi: 10.1016/j.livres.2025.01.004

Figure Lengend Snippet: Effects of BFA and TM on the mRNA and protein levels of apoptosis-related factors (PARP-1, caspase-12, caspase-3, and SCD1). HepG2 cells were treated with BFA (0.25 mg/L) and TM (1 mg/L), either individually or in combination, for 24 h. The relative mRNA levels of (A) PARP-1 , (B) caspase-12 , (C) caspase-3, and (D) SCD1 were determined by qRT-PCR. The protein levels of (E) cleaved PARP-1, (F) caspase-12, (G) full-length and cleaved caspase-3, and (H) SCD1 were determined by Western blotting. Data are presented as means ± SD from three independent experiments performed in triplicate. ∗ P < 0.05 vs. control group; # P < 0.05 vs. DMSO (0.1% v/v, vehicle control) group; a P < 0.05 vs. BFA (0.25 mg/L) group. Abbreviations: BFA, brefeldin A; DMSO, dimethyl sulfoxide; PARP-1, poly (ADP-ribose) polymerase-1; qRT-PCR, quantitative real-time polymerase chain reaction; SCD1, stearoyl-CoA desaturase 1; SD, standard deviation; TM, tunicamycin.

Article Snippet: Rabbit anti-human SCD1 , Protein Tech, China , 28678-1-AP , 1:1000.

Techniques: Quantitative RT-PCR, Western Blot, Control, Real-time Polymerase Chain Reaction, Standard Deviation

Journal: International Journal of Biological Sciences

Article Title: ETS1-mediated Regulation of SOAT1 Enhances the Malignant Phenotype of Oral Squamous Cell Carcinoma and Induces Tumor-associated Macrophages M2-like Polarization

doi: 10.7150/ijbs.93815

Figure Lengend Snippet: Primers for qPCR

Article Snippet: The following antibodies were used for western blot: mouse monoclonal anti-human β-actin antibody (#AF7018, Affinity, 1:4000), rabbit anti-human SOAT1 antibody (#A6311, ABclonal, 1:4000), mouse anti-human ETS1 antibody (#66598-1-Ig, Proteintech, 1:4000), rabbit anti-human SREBP1 antibody (#347061, Zenbio, 1:1000), rabbit anti-human SCD1 antibody (#28678-1-AP, Proteintech, 1:2000), rabbit anti-human p-PI3K antibody (#AF3242, Affinity, 1:1000), mouse anti-human PI3K antibody (#60225-1-Ig, Proteintech, 1:1000), mouse anti-human p-AKT antibody (#66444-1-Ig, Proteintech, 1:5000), mouse anti-human AKT antibody (#YM3618, ImmunoWay, 1:1000), rabbit anti-human p-mTOR antibody (#381557, Zenbio, 1:1000), rabbit anti-human mTOR antibody (#380411, Zenbio, 1:1000), goat anti-rabbit antibody (#S0001, Affinity, 1:6000) and goat anti-mouse antibody (#S0002, Affinity, 1:6000).

Techniques:

Journal: Nature cancer

Article Title: Metabolic adaptation of acute lymphoblastic leukemia to the central nervous system microenvironment is dependent on Stearoyl CoA desaturase

doi: 10.1038/s43018-020-00115-2

Figure Lengend Snippet: (A) Quantitative PCR validation of top ranked genes from RNA-Seq in n=5 patient derived xenografts with t(12;21) ETV6-RUNX1 (TEL-AML1) translocation or t(4;11) MLL-AFF1 (MLL-AF4)] translocation. Results are normalized to 36B4 human housekeeping gene and presented as Log2 fold change enrichment comparing CNS to spleen. p (two tailed) = One sample T and Wilcoxon test. (B) Gene expression of SCD1 from RNAseq data deposited in public databases. Left: Samples of BM of patients at diagnosis and relapse and CNS at relapse, unpaired analysis. Right: Patient-derived xenograft samples established by transplantation of patients’ ALL cells onto NSG mice. SCD1 expression in cells isolated from paired CNS and spleen is shown in the graph. FDR – False discovery rate. (C) Intracellular staining of SCD1 in cells from the BM (green) and CNS (grey) of a mouse transplanted with 018z cells. The peaks are relative to the percentage of human CD19+ cells normalized to mode.

Article Snippet: Fifty μg of total protein lysate were loaded on a 12% Polyacrylamide gel and incubated overnight with rabbit anti-human monoclonal SCD1 antibody 1:2000 (SCD1 (C 12 H 5 ) Rabbit mAb #2794, Cell Signaling) and vinculin (Vinculin Antibody #4650, Cell Signaling) at 4°C.

Techniques: Real-time Polymerase Chain Reaction, RNA Sequencing Assay, Derivative Assay, Translocation Assay, Two Tailed Test, Expressing, Transplantation Assay, Isolation, Staining

Journal: Nature cancer

Article Title: Metabolic adaptation of acute lymphoblastic leukemia to the central nervous system microenvironment is dependent on Stearoyl CoA desaturase

doi: 10.1038/s43018-020-00115-2

Figure Lengend Snippet: (A) SCD1 gene expression levels following SCD1 overexpression in 018z cells. (B) Western blot of SCD1 protein after overexpression in 018z cells. (C)I-II Ratio of relative levels of oleoyl-CoA/stearoyl-CoA and of palmitoleoyl-CoA/palmitoyl-CoA in control (CTL) and SCD1-high 018z cells. p=Student’s t-test. (D) Human leukemia burden in 018z xenograft model. Total amount of leukemic cells in CNS, BM and spleen of NSG mice xenografted with human 018z ALL cell line overexpressing SCD1 (SCD1-high) or control (CTL) GFP+ at the time of sacrifice. (E) Human leukemia burden in REH xenograft model. Total amount of leukemic cells in CNS, BM and spleen of NSG mice xenografted with human REH ALL cell lines overexpressing SCD1 (SCD1-high) or control (CTL) GFP+ at the time of sacrifice. p=paired Student’s t-test. (F) Competition assay in vivo: FACS plots of cells injected (INPUT) and cells isolated from the CNS of the mice at sacrifice (OUTPUT). Top: GFP+ control cells transduced with a GFP-carrying lentiviral backbone and mCherry+ control cells transduced with a mCherry carrying lentiviral backbone injected in a ratio of 1:1. Bottom: GFP+ SCD1 overexpressing (SCD1-high) cells and mCherry+ control cells transduced with a mCherry carrying lentiviral backbone injected in a ratio of 1:1. (G) Ratio of total number of SCD1-high 018z cells (GFP+) to control 018z cells (mCherry+) in CNS, BM and spleen of NSG mice transplanted with a mixture of the two cell types in a ratio of 1:1. The backbone vector for SCD1 overexpression was used as control vector. p=paired Student’s t-test.

Article Snippet: Fifty μg of total protein lysate were loaded on a 12% Polyacrylamide gel and incubated overnight with rabbit anti-human monoclonal SCD1 antibody 1:2000 (SCD1 (C 12 H 5 ) Rabbit mAb #2794, Cell Signaling) and vinculin (Vinculin Antibody #4650, Cell Signaling) at 4°C.

Techniques: Expressing, Over Expression, Western Blot, Competitive Binding Assay, In Vivo, Injection, Isolation, Transduction, Plasmid Preparation

Journal: Nature cancer

Article Title: Metabolic adaptation of acute lymphoblastic leukemia to the central nervous system microenvironment is dependent on Stearoyl CoA desaturase

doi: 10.1038/s43018-020-00115-2

Figure Lengend Snippet: (A) Western blot analysis of SCD1 after CRISPR-Cas9 gene ablation in 018z cells. Each lane represents a different gRNA used for the initial screening. gRNA4 decreased SCD1 protein expression while gRNA1-3 did not affect its levels. (B) Gene expression level of SCD1 after CRISPR-Cas9 knockout in 018z cells by gRNA4. (C)I-II Ratio of relative levels of oleoyl-CoA/stearoyl-CoA and of palmitoleoyl-CoA/palmitoyl-CoA in control (CTL) and SCD1-low 018z cells. p=Student’s t-test (D) In vitro proliferation of SCD1-low and control (CTL) 018z cells after 96 hours in in medium supplemented with 10% lipidated or delipidated FBS. The dotted line represents the initial number of cells plated at T0. p=two-way ANOVA. (E) Human leukemia burden in 018z xenograft model. Total amount of leukemic cells in CNS, BM and spleen of NSG mice xenografted with human 018z ALL cell lines SCD1-low or control GFP at the time of sacrifice. p=paired Student’s t-test. (F) Intracellular staining of SCD1 in human cells isolated from the BM of mice transplanted with SCD1-low and control (CTL) 018z cells. The scramble vector for SCD1 downregulation was used as control vector. The peaks are relative to the percentage of human CD19+ cells normalized to mode.

Article Snippet: Fifty μg of total protein lysate were loaded on a 12% Polyacrylamide gel and incubated overnight with rabbit anti-human monoclonal SCD1 antibody 1:2000 (SCD1 (C 12 H 5 ) Rabbit mAb #2794, Cell Signaling) and vinculin (Vinculin Antibody #4650, Cell Signaling) at 4°C.

Techniques: Western Blot, CRISPR, Expressing, Knock-Out, In Vitro, Staining, Isolation, Plasmid Preparation

Journal: Nature cancer

Article Title: Metabolic adaptation of acute lymphoblastic leukemia to the central nervous system microenvironment is dependent on Stearoyl CoA desaturase

doi: 10.1038/s43018-020-00115-2

Figure Lengend Snippet: In vitro proliferation of “SCD1-high”, “SCD1-low” and matching control (CTL) 018z cells after seeding 0.5x106 cells/well for 96 hours in medium supplemented with 10% lipidated (A-B) or delipidated (C-D) FBS treated with 1μM of the SCD1 inhibitor SW203668 or vehicle (DMSO).The dotted line represents the initial number of cells plated at T0. p=two-way ANOVA. (D) GFP+mCherry+FFLuc+ 018z cells were injected intravenously to NSG mice and treated from day 1 to day 10 with the SCD1 inhibitor SW203668 or vehicle (n=5 group). Representative bioluminescence of the tumor load at the time of sacrifice in three different pairs of mice, top: vehicle treated mice; bottom: drug treated mice. Decrease in the tumor load is clear in the spine and the skull area (CNS – marked with white box). Total amount of leukemic cells in CNS (E) and BM (F) of NSG mice xenografted with human GFP+mCherry+FFLuc+ 018z and treated with SW203668 for 10 days at the time of sacrifice. The backbone vector for SCD1 overexpression was used as control vector for SCD1-high and the scramble vector was used as control for SCD1-low. BM – bone marrow. p=Student’s t-test.

Article Snippet: Fifty μg of total protein lysate were loaded on a 12% Polyacrylamide gel and incubated overnight with rabbit anti-human monoclonal SCD1 antibody 1:2000 (SCD1 (C 12 H 5 ) Rabbit mAb #2794, Cell Signaling) and vinculin (Vinculin Antibody #4650, Cell Signaling) at 4°C.

Techniques: In Vitro, Injection, Plasmid Preparation, Over Expression

Journal: Nature cancer

Article Title: Metabolic adaptation of acute lymphoblastic leukemia to the central nervous system microenvironment is dependent on Stearoyl CoA desaturase

doi: 10.1038/s43018-020-00115-2

Figure Lengend Snippet: Human leukemia burden in PDXs. Cells from 4 different PDXs were injected intravenously into NSG mice. Xenografted mice were treated daily from day 7 with the SCD1 inhibitor SW203668 or vehicle (n=5 group) at 20 mg/kg. The total amounts of leukemic cells in CNS and BM of NSG mice at the time of sacrifice are plotted in the graphs (A-D). p=Student’s t-test.

Article Snippet: Fifty μg of total protein lysate were loaded on a 12% Polyacrylamide gel and incubated overnight with rabbit anti-human monoclonal SCD1 antibody 1:2000 (SCD1 (C 12 H 5 ) Rabbit mAb #2794, Cell Signaling) and vinculin (Vinculin Antibody #4650, Cell Signaling) at 4°C.

Techniques: Injection